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aav9 paav cmv hi cre gfp wpre addgene addgene  (Addgene inc)


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    Structured Review

    Addgene inc aav9 paav cmv hi cre gfp wpre addgene addgene
    Aav9 Paav Cmv Hi Cre Gfp Wpre Addgene Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+cre+gfp/AAV+pCAG-FLEX-EGFP-WPRE+(Plasmid+%2351502)/pm41860867-130-62-64
    Average 96 stars, based on 189 article reviews
    aav9 paav cmv hi cre gfp wpre addgene addgene - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Division of labor among H3K4 methyltransferases defines distinct facets of homeostatic plasticity.
    Article Snippet: .. pCAG-Cre:GFP was a gift from Dr. Connie Cepko (Addgene plasmid #13776). .. 69 The retroviral pMSCVhyg plasmid expressing wild type (WT) histone H3.3 and Lys4-to-Met mutant expressing plasmids were gifts from Dr. Kai Ge (Addgene plasmid # 128740, # 128741).

    Article Title: hESCs‐derived Organoids Achieve Liver Zonation Features through LSEC Modulation
    Article Snippet: .. Following confirmation, the cells were further transfected with pCAG‐Cre: GFP (Addgene plasmid ID:13 776) to facilitate recombination and removal of the Hyg segment. ..

    Article Title: A human CAGinSTEM platform for decoding HTT repeats' somatic instability links CAG interruption to HD pathology in neurons.
    Article Snippet: .. Generation of the CAGinSTEM platform with increasing CAG length and/or modified CAG stretch composition within HTT exon1 To generate the entire platform, 2 × 106 cells of the RMCE-H9 parental clones (#01, #04, #25) were nucleofected with 1 μg of pUC57_HDR_RMCE plasmid carrying a specific exon1 variant and 1 μg of pCAG-Cre:GFP (Addgene) by using Nucleofector kit (Lonza), in Nucleofector II (Amaxa biosystems) using the B-016 program. ..

    Article Title: A human CAGinSTEM platform for decoding HTT repeats’ somatic instability links CAG interruption to HD pathology in neurons
    Article Snippet: .. To generate the entire platform, 2 × 106 cells of the RMCE-H9 parental clones (#01, #04, #25) were nucleofected with 1 μg of pUC57_HDR_RMCE plasmid carrying a specific exon1 variant and 1 μg of pCAG-Cre:GFP (Addgene) by using Nucleofector kit (Lonza), in Nucleofector II (Amaxa biosystems) using the B-016 program. ..

    Article Title: Empirical validation of ephaptic coupling in printed human neural circuits
    Article Snippet: .. The mRNA was prepared by amplifying the Cre DNA via PCR using the following primers: T7-Cre-forward: 5’-GCTAATACGACTCACTATAGGGACAGGCCACCATGGCCAATTTACTGA-3’ and T7-Cre-reverse: 5’-TCATTACGGTCCATCGCCATCTTCCAGCAGGCGCACCATT-3’ from the pCAG-Cre:GFP plasmid. pCAG-Cre:GFP was a gift from Connie Cepko (Addgene plasmid # 13776) . .. The HiScribe ® T7 ARCA mRNA Kit (with tailing) (New England Biolabs) was used to create mRNA and subsequently cleaned up with the Monarch ® Spin RNA Cleanup Kit (50 μg) (New England Biolabs).

    Selection:

    Article Title: Isogenic cortical organoids enable precision targeting of APP variant-specific pathways in Alzheimer’s disease
    Article Snippet: Successfully targeted cells were selected with 1 μg/mL puromycin (Life Technologies, A1113802) for 7 days. .. To excise the selection cassette, cells were nucleofected with pCAG-Cre:GFP (Addgene #13776), and 24 h later, GFP-positive and mRuby-negative cells were enriched by fluorescence-activated cell sorting (FACS). ..

    Fluorescence:

    Article Title: Isogenic cortical organoids enable precision targeting of APP variant-specific pathways in Alzheimer’s disease
    Article Snippet: Successfully targeted cells were selected with 1 μg/mL puromycin (Life Technologies, A1113802) for 7 days. .. To excise the selection cassette, cells were nucleofected with pCAG-Cre:GFP (Addgene #13776), and 24 h later, GFP-positive and mRuby-negative cells were enriched by fluorescence-activated cell sorting (FACS). ..

    FACS:

    Article Title: Isogenic cortical organoids enable precision targeting of APP variant-specific pathways in Alzheimer’s disease
    Article Snippet: Successfully targeted cells were selected with 1 μg/mL puromycin (Life Technologies, A1113802) for 7 days. .. To excise the selection cassette, cells were nucleofected with pCAG-Cre:GFP (Addgene #13776), and 24 h later, GFP-positive and mRuby-negative cells were enriched by fluorescence-activated cell sorting (FACS). ..

    Transfection:

    Article Title: hESCs‐derived Organoids Achieve Liver Zonation Features through LSEC Modulation
    Article Snippet: .. Following confirmation, the cells were further transfected with pCAG‐Cre: GFP (Addgene plasmid ID:13 776) to facilitate recombination and removal of the Hyg segment. ..

    Modification:

    Article Title: A human CAGinSTEM platform for decoding HTT repeats' somatic instability links CAG interruption to HD pathology in neurons.
    Article Snippet: .. Generation of the CAGinSTEM platform with increasing CAG length and/or modified CAG stretch composition within HTT exon1 To generate the entire platform, 2 × 106 cells of the RMCE-H9 parental clones (#01, #04, #25) were nucleofected with 1 μg of pUC57_HDR_RMCE plasmid carrying a specific exon1 variant and 1 μg of pCAG-Cre:GFP (Addgene) by using Nucleofector kit (Lonza), in Nucleofector II (Amaxa biosystems) using the B-016 program. ..

    Clone Assay:

    Article Title: A human CAGinSTEM platform for decoding HTT repeats' somatic instability links CAG interruption to HD pathology in neurons.
    Article Snippet: .. Generation of the CAGinSTEM platform with increasing CAG length and/or modified CAG stretch composition within HTT exon1 To generate the entire platform, 2 × 106 cells of the RMCE-H9 parental clones (#01, #04, #25) were nucleofected with 1 μg of pUC57_HDR_RMCE plasmid carrying a specific exon1 variant and 1 μg of pCAG-Cre:GFP (Addgene) by using Nucleofector kit (Lonza), in Nucleofector II (Amaxa biosystems) using the B-016 program. ..

    Article Title: A human CAGinSTEM platform for decoding HTT repeats’ somatic instability links CAG interruption to HD pathology in neurons
    Article Snippet: .. To generate the entire platform, 2 × 106 cells of the RMCE-H9 parental clones (#01, #04, #25) were nucleofected with 1 μg of pUC57_HDR_RMCE plasmid carrying a specific exon1 variant and 1 μg of pCAG-Cre:GFP (Addgene) by using Nucleofector kit (Lonza), in Nucleofector II (Amaxa biosystems) using the B-016 program. ..

    Variant Assay:

    Article Title: A human CAGinSTEM platform for decoding HTT repeats' somatic instability links CAG interruption to HD pathology in neurons.
    Article Snippet: .. Generation of the CAGinSTEM platform with increasing CAG length and/or modified CAG stretch composition within HTT exon1 To generate the entire platform, 2 × 106 cells of the RMCE-H9 parental clones (#01, #04, #25) were nucleofected with 1 μg of pUC57_HDR_RMCE plasmid carrying a specific exon1 variant and 1 μg of pCAG-Cre:GFP (Addgene) by using Nucleofector kit (Lonza), in Nucleofector II (Amaxa biosystems) using the B-016 program. ..

    Article Title: A human CAGinSTEM platform for decoding HTT repeats’ somatic instability links CAG interruption to HD pathology in neurons
    Article Snippet: .. To generate the entire platform, 2 × 106 cells of the RMCE-H9 parental clones (#01, #04, #25) were nucleofected with 1 μg of pUC57_HDR_RMCE plasmid carrying a specific exon1 variant and 1 μg of pCAG-Cre:GFP (Addgene) by using Nucleofector kit (Lonza), in Nucleofector II (Amaxa biosystems) using the B-016 program. ..

    Polymerase Chain Reaction:

    Article Title: Empirical validation of ephaptic coupling in printed human neural circuits
    Article Snippet: .. The mRNA was prepared by amplifying the Cre DNA via PCR using the following primers: T7-Cre-forward: 5’-GCTAATACGACTCACTATAGGGACAGGCCACCATGGCCAATTTACTGA-3’ and T7-Cre-reverse: 5’-TCATTACGGTCCATCGCCATCTTCCAGCAGGCGCACCATT-3’ from the pCAG-Cre:GFP plasmid. pCAG-Cre:GFP was a gift from Connie Cepko (Addgene plasmid # 13776) . .. The HiScribe ® T7 ARCA mRNA Kit (with tailing) (New England Biolabs) was used to create mRNA and subsequently cleaned up with the Monarch ® Spin RNA Cleanup Kit (50 μg) (New England Biolabs).



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